Signalling
ComX

Part:BBa_K2273003:Design

Designed by: Jonathan Hammer   Group: iGEM17_TU_Dresden   (2017-09-25)


ComX pheromone for competence development in Bacillus subtilis


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design

This part was generated with RFC10 Suffix and Prefix. It encodes for the srfA promoter that is part of the ComX quorum response of B. subtilis and was designed for the subproject "Communication" by the [http://2017.igem.org/Team:TU_Dresden/Project/Communication iGEM Team TU Dresden 2017 (EncaBcillus - It's a trap!)].

Source

The comX gene was amplified via PCR from the B. subtilis wild type W168 genome using the primers listed below.

comX fwd RFC10 gatcGAATTCGCGGCCGCTTCTAGAAGGAGGTCAAAAATGCAAGACCTAATTAACTAC
comX rev RFC10 gatcCTGCAGCGGCCGCTACTAGTTTAATCACCCCATTGACGGG

References

Jung J, Yu KO, Ramzi AB, Choe SH, Kim SW, Han SO (2012) Improvement of surfactin production in Bacillus subtilis using synthetic wastewater by overexpression of specific extracellular signaling peptides, comX and phrC. Biotechnol Bioeng. 109(9):2349-56. doi: 10.1002/bit.24524. Epub 2012 Apr 24. PubMed

Hamoen LW, Venema G, Kuipers OP (2003) Controlling competence in Bacillus subtilis: shared use of regulators. Microbiology. 149(Pt 1):9-17 PubMed