Reporter
Part:BBa_K2272011:Design
Designed by: Eric Joshua Wang Group: iGEM17_Virginia (2017-10-22)
pBAD N.E. TAT-GFP-RFP
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 125
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 65
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 222
Illegal AgeI site found at 1579
Illegal AgeI site found at 1691 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 919
Design Notes
This construct was desgined to validate the function of the signal peptide.
Source
The GFP and RFP coding sequence was from the iGEM 2017 distribution kit.