Reporter

Part:BBa_K2272010:Design

Designed by: Eric Joshua Wang   Group: iGEM17_Virginia   (2017-10-22)


pBAD P.D. TAT-GFP-RFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 125
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 65
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 171
    Illegal NgoMIV site found at 219
    Illegal NgoMIV site found at 225
    Illegal NgoMIV site found at 240
    Illegal AgeI site found at 1749
    Illegal AgeI site found at 1861
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 952


Design Notes

This construct was desgined to validate the function of the signal peptide.


Source

The GFP and RFP coding sequence was from the iGEM 2017 distribution kit.

References