Reporter

Part:BBa_K2272009:Design

Designed by: Eric Joshua Wang   Group: iGEM17_Virginia   (2017-10-22)


pBAD N.E. TAT-RFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 125
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 65
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 222
    Illegal AgeI site found at 853
    Illegal AgeI site found at 965
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This construct was desgined to validate the function of the signal peptide.


Source

The RFP coding sequence was from the iGEM 2017 distribution kit.

References