Coding

Part:BBa_K2272002:Design

Designed by: Eric Joshua Wang   Group: iGEM17_Virginia   (2017-10-21)


HAO (hydroxylamine oxidoreductase)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 339
    Illegal XhoI site found at 1903
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 355
    Illegal NgoMIV site found at 832
    Illegal NgoMIV site found at 836
    Illegal NgoMIV site found at 2604
    Illegal NgoMIV site found at 2994
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 259
    Illegal BsaI.rc site found at 307
    Illegal BsaI.rc site found at 874
    Illegal BsaI.rc site found at 3034


Design Notes

This oxidoreductase works closely with cytochrome(cyt) and ammonium monooxygenase(AMO) to complete the nitrification process. In our project(http://2017.igem.org/Team:Virginia), we designed our final construct with all three protein coding sequences.


Source

Characterization of the gene encoding hydroxylamine oxidoreductase in Nitrosomonas europaea. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC205074/

Part:BBa_K1067004 iGEM13_DTU-Denmark https://parts.igem.org/Part:BBa_K1067004

References