Coding

Part:BBa_K2271106:Design

Designed by: Manuel Lentzen   Group: iGEM17_Cologne-Duesseldorf   (2017-10-27)


PTS1 variant P3


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The peroxisomal targeting signal 1 supposedly consists of 12 amino acids(Erdmann et al.,2015). We did research on the amino acid composition naturally occuring in Pts1 sequences (Lametschwandter et al., 1998) and designed our Pts1 sequences with respect to that, just modifying the last four amino acids.

References

Structural Insights into Cargo Recognition by the Yeast PTS1 Receptor - Erdmann et al., 2015
The Difference in Recognition of Terminal Tripeptides as Peroxisomal Targeting Signal 1 between Yeast and Human Is Due to Different Affinities of Their Receptor Pex5p to the Cognate Signal and to Residues Adjacent to It - Lametschwandter et al., 1998