Coding

Part:BBa_K2271067:Experience

Designed by: Jason Marcel Müller, Manuel Lentzen   Group: iGEM17_Cologne-Duesseldorf   (2017-10-27)

Applications of BBa_K2271067

Figure 1:PEX5 knock out strain, transformed with the R19 variant, mTurqouise tagged with the PTS1 and the PEX13 membrane anchor fused to mRuby.
Figure 2:PEX5 knock out strain, transformed with the R19 variant, mTurqouise tagged with the PTS1 and the PEX13 membrane anchor fused to mRuby.

The figures 1 and 2 show the functionality of our Pex5 variant R19. R19 with PTS1* leads to clear import whereas the controls with the wild type PTS1 and the wild type Pex5 with PTS1* do not show any signs for import into the peroxisome.

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