Reporter

Part:BBa_K2271026:Design

Designed by: Remy Bastiaan Tjeng   Group: iGEM17_Cologne-Duesseldorf   (2017-10-27)


roGFP2 fused to PTS1 and Pex13-mRuby fusion construct


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal suffix found in sequence at 3560
    Illegal BglII site found at 730
    Illegal BglII site found at 2459
    Illegal BglII site found at 2496
    Illegal BamHI site found at 1456
    Illegal XhoI site found at 1492
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Peroxisomal targeting sequence PTS1 fused to the carboxyl tail of roGFP2 and N-terminal fusion of the fluorescence protein mRuby to peroxisomal membrane protein Peroxin13.


Source

IDT Synthese of roGFP2 and peroxin. Multicassette construct gained from Goldengate cloning with yeast toolbox from prof. John Dueber.

References