Reporter

Part:BBa_K2271024:Design

Designed by: Remy Bastiaan Tjeng   Group: iGEM17_Cologne-Duesseldorf   (2017-10-27)


roGFP2 redox sensing green flourescent protein with PTS1 for yeast expression


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 880
    Illegal BamHI site found at 1606
    Illegal XhoI site found at 1642
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The promotor (pCCW12; strong), c-terminal PTS1 tagged roGFP2 and terminator (tENO1) are codon optimized for yeast.

Source

Synthese from IDT. DNA sequence from Gutscher, M., et al. (2008). "Real-time imaging of the intracellular glutathione redox potential." Nat Methods 5(6): 553-559. Yeast codon optimized. Final part gained from Goldengate cloning with Yeast toolbox from John Dueber.

References

(Gutscher M. et al., 2008)