Signalling
Part:BBa_K2271016:Design
Designed by: Jason Marcel Müller, Manuel Lentzen Group: iGEM17_Cologne-Duesseldorf (2017-10-27)
PTS1 variant P*
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The peroxisomal targeting signal 1 supposedly consists of 12 amino acids(Erdmann et al.,2015). We did research on the amino acid composition naturally occuring in Pts1 sequences (Lametschwandter et al., 1998) and designed our Pts1 sequences with respect to that, just modifying the last four amino acids.
References
Structural Insights into Cargo Recognition by the Yeast PTS1
Receptor - Erdmann et al., 2015
The Difference in Recognition of Terminal Tripeptides as
Peroxisomal Targeting Signal 1 between Yeast and Human
Is Due to Different Affinities of Their Receptor Pex5p to the
Cognate Signal and to Residues Adjacent to It - Lametschwandter et al., 1998