Coding

Part:BBa_K2271010

Designed by: Eric Behle   Group: iGEM17_Cologne-Duesseldorf   (2017-10-27)


Pex34p with ScCCW12 promoter

This part contains the coding sequence for the peroxin Pex34 with the strong constitutive promoter ScSSW12 and the terminator ScADH1. The sequence is codon-optimized for S. cerevisiae.

Usage and Biology

Pex34p is a peroxisomal integral membrane protein. It can act independently as well as in combination with Pex11p, Pex25p, and Pex27p to control the peroxisome morphology and population. Pex34p is suggested to directly influence peroxisome proliferation as well as constitutive peroxisome division. Overexpression of Pex34 leeds to a high number of large peroxisomes.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal suffix found in sequence at 1139
    Illegal BglII site found at 701
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None