Reporter
sfGFP(Bs)

Part:BBa_K2270010

Designed by: Overkamp, W. et al.   Group: iGEM17_AQA_Unesp   (2017-10-18)


superfolding GFP for Lactococcus lactis

Superfolding GFP codon-optimized for Bacillus subtilis. However, exhibits strong fluorescense signal in Lactococcus lactis. Used as protein report in L. lactis.

Usage and Biology

We (AQA_Unesp 2017) characterized the gene expression of sfGFP(Bs) during Lactococcus lactis growth under control of the usp45 promoter.


For more Details visit our [http://2017.igem.org/Team:AQA_Unesp/Results webpage]

Figure 1. Pellet of Lactococcus lactis cremoris cloned with Pusp45_GFP genes exited by a blue led transilluminator with filter.

Characterization

Figure 2. Growth curve and fluorescence from Lactococcus lactis cremoris cloned with Pusp45_GFP genes.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 13


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Categories
Parameters
None