Regulatory

Part:BBa_K2269001

Designed by: José Álvaro Ballesteros González   Group: iGEM17_Valencia_UPV   (2017-09-06)


Register Assembly 2/3 (Pr-1a)

Middle part for assembly of site-specific recombination based Registers. Composed of the PR-1a inverted sequence and the Mtb terminator flanked by two opposing PhiC31 site-specific recombination sites (attR and attL).

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 54
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 54
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 371
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 54
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 54
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None