Part:BBa_K2259092

Minimal base vector for SynORI system building

This is the backbone base vector 2.0, which contains no insert. See Part:BBa_K2259081 for the full vector.

Engineering an improved, functional base vector 2.0 is crucial for the SynORI framework.

Building a modular synthetic origin of replication is easiest when working in the biobrick region. But for synthetic ORI this is only possible if there is no other origin of replication in the rest of the vector. Base vector 2.0 provides this, as it has its pUC replicon in biobrick site.

One can then replace the pUC origin of replication to SynORI system parts. Once the modular SynORI system is built, it can be transferred to another plasmid location and biobricks are then free to use for any task.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal EcoRI site found at 1349
  Illegal NheI site found at 177
  Illegal NheI site found at 1126
  Illegal SpeI site found at 2
  Illegal PstI site found at 16
  Illegal NotI site found at 9
  Illegal NotI site found at 1355
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal EcoRI site found at 1349
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal prefix found at 1349
  Illegal suffix found at 2
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal prefix found at 1349
  Plasmid lacks a suffix.
  Illegal XbaI site found at 1364
  Illegal SpeI site found at 2
  Illegal PstI site found at 16
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  

About SynORI

SynORI is a framework for multi-plasmid systems created by Vilnius-Lithuania 2017 which enables quick and easy workflow with multiple plasmids, while also allowing to freely pick and modulate copy number for every unique plasmid group! Read more about [http://2017.igem.org/Team:Vilnius-Lithuania SynORI here]!

This part in SynORI

Engineering an improved, functional base vector 2.0 was crucial for the SynORI framework because building a synthetic origin of replication required an empty biobrick site and no origin of replication in the backbone. One can insert SynORI system parts into the backbone to build their own custom origin of replication. Once the modular SynORI system is constructed, it can be transferred to another plasmid location and biobrick sites are then free to use for other projects as required.

See [http://2017.igem.org/Team:Vilnius-Lithuania Vilnius-Lithuania 2017 team wiki] for more insight about our synthetic origin of replication (SynORI).