Part:BBa_K2259035:Design

Toehold activated beta-neomycin (SynORI framework)

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 7
  Illegal NheI site found at 30
  
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 584
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal SapI.rc site found at 433
  Illegal SapI.rc site found at 643
  

Design Notes

Mutations for group selection had to be carefully tailored in order not to change secondary structure of RNA I.

Source

This part is a modified version of wildtype ColE1 RNA I taken from addgene.