Coding

Part:BBa_K2259010:Design

Designed by: Laurynas Karpus   Group: iGEM17_Vilnius-Lithuania   (2017-10-01)


Rop protein - global copy number inhibitor (SynORI framework)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Only the coding sequence of Rop was used from pBR322 plasmid, as the wild-type promoter of rop is relatively poorly characterised.


Source

Sequence was acquired from addgene pBR322 plasmid

References