RNA
Part:BBa_K2259007:Design
Designed by: Laurynas Karpus Group: iGEM17_Vilnius-Lithuania (2017-10-01)
SynORI framework RNA I - Replication Inhibitor (Group B-GC)
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Sequences was taken from wildtype ColE1 origin ignoring the RNA II segment for RNA I to be controlled independedly.
Source
Wildtype ColE1 sequence was taken from addgene, mutated and ordered from IDT.