Project
RNA II (C)

Part:BBa_K2259002:Design

Designed by: Laurynas Karpus   Group: iGEM17_Vilnius-Lithuania   (2017-10-01)


SynORI framework RNA II - Replication Initiator (Group C)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Sequence has been mutated to inactivate RNR 1 from the anti-sense strand.


Source

Wildtype ColE1 sequence was taken from addgene, mutated and ordered from IDT.

References