Composite

Part:BBa_K2258004

Designed by: Jyothish S, Yash Jawale   Group: iGEM17_IISER-Pune-India   (2017-10-27)


pL_pLeft_AmilCP

This construct expresses AmilCP constitutively under pL-pLeft hybrid promoter

Usage and Biology

This part will act as constitutively express AmilCP in both E.coli and Mycobacterium under the control of pL-pLeft hybrid promoter.


Reference

  1. PL Expression System : A Prokaryotic Expression System, Invitrogen manual
  2. Transcriptional regulation of repressor synthesis in mycobacteriophage L5, Chadd E. Nesbit, Margaret E. Levin, Mary K. Donnelly-Wu and Graham F. HatfuU
  3. Diversity and Evolution of Coral Fluorescent Proteins, Naila O. Alieva, Karen A. Konzen, Steven F. Field, Ella A. Meleshkevitch, Marguerite E. Hunt, Victor Beltran-Ramirez, David J. Miller, Jörg Wiedenmann, Anya Salih, Mikhail V. Matz

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//chassis/prokaryote
Parameters
chassisE.coli/Mycobacterium
device_typeReporter