Regulatory
YJ-shuttle

Part:BBa_K2258000

Designed by: Yash Jawale   Group: iGEM17_IISER-Pune-India   (2017-10-27)


pL and Pleft Hybrid promoter

Acts as shuttle promoter, since pL from Lambda phage allows expression in Escherichia coli and Pleft from Mycobacterio phage allows expression in Mycobacterium.

Usage and Biology

Acts as shuttle promoter, since pL from Lambda phage allows expression in Escherichia coli and Pleft from Mycobacterio phage allows expression in Mycobacterium.

Reference

  1. PL Expression System : A Prokaryotic Expression System, Invitrogen manual
  2. Transcriptional regulation of repressor synthesis in mycobacteriophage L5, Chadd E. Nesbit, Margaret E. Levin, Mary K. Donnelly-Wu and Graham F. HatfuU


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//awards/basic_part
//chassis/bacteriophage
//chassis/prokaryote
Parameters
biologyconstitutive promoter
directionForward
targetE.coli/Mycobacterium