Part:BBa_K2255002:Design

RBS + Thioesterase

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

Design Notes

This Thioestherase^[1] is necessary to the production of 2-cis-decenenoic acid by Pseudomonas aeruginosa, so we search its DNA sequence in the genome to create our first biobrick. This enzyme gene is on a operon along the other gene crucial for the formation of 2-cis-decenenoic acid.

We took the gene coding for the right enzyme and optimized its sequence for a production in E.coli. Then, a ribosome binding site (RBS) was added to the sequence in order to make an operon with BBa_K2255000 and BBa_K2255001. Finaly, a Rfc10 prefix and sufix was added.

Source

This sequence came from Pseudomonas aeruginosa.

References

↑ Amari, D. T., Marques, C. N. H. & Davies, D. G. The Putative Enoyl-Coenzyme A Hydratase DspI Is Required for Production of the Pseudomonas aeruginosa Biofilm Dispersion Autoinducer cis-2-Decenoic Acid. J. Bacteriol. 195, 4600–4610 (2013).

[1] Amari, D. T., Marques, C. N. H. & Davies, D. G. The Putative Enoyl-Coenzyme A Hydratase DspI Is Required for Production of the Pseudomonas aeruginosa Biofilm Dispersion Autoinducer cis-2-Decenoic Acid. J. Bacteriol. 195, 4600–4610 (2013).

[1]