Coding

Part:BBa_K2250000

Designed by: Shuailin Li   Group: iGEM17_Tsinghua-A   (2017-10-18)


This is the coding sequence of RafD, a invertase which can catalyze sucrose to glucose and fructose.

It is the coding sequence of RafD, a invertase which can catalyze the hydrolysis of sucrose to glucose and fructose so that the bacteria can use sucrose in the medium. We link this protein before the signal peptide of HlyA to form part BBa_K2250002 so that it can be secreted outside the cell, which can make not only the producer use sucrose, but also other bacteria which can not synthesize this protein. Detailed information can be seen at main page of BBa_K2250003.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 1356
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 97
    Illegal NgoMIV site found at 968
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 799


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Categories
Parameters
None