Coding
Part:BBa_K2244004:Design
Designed by: Chen Hong Group: iGEM17_SSTi-SZGD (2017-10-22)
mheI
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 591
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 250
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Gene mheI (Genebank No: KX698097.2) was commercially synthesized and codon optimized for expression in E. coli chassis.
Source
MBC hydrolase gene (mheI) is derived from carbendazim-degrading strain Mycobacterium sp. SD-4 and is a genomic DNA gene sequence. mheI gene is also evolutionary conserved in many other bacteria strains, such as Nocardioides sp. SG-4G, and Rhodococcus erythropolis.