Part:BBa_K2242521
placI+lacI+pTAC+CmCR
Ethyl (S)-4-chloro-3-hydroxybutanoate [(S)-CHBE] is a key intermediate for the production of chiral drugs, including choles- terol-lowering HMG-CoA reductase inhibitors such as Lipitor. Therefore, a more practical way to synthesize highly optical active of (S)-CHBE (>99.9% ee) is of great interest. Compared with conventional chemical synthesis, the asymmetric bioreduction of ethyl 4-chloro-3-oxobutanoate (COBE), which is inexpensive and easily synthesized, is an economical approach to the production of (S)-CHBE. From recently research, we find that there is an enzyme can efficiently catalyze this reduction reaction. In addition, this reductase is a NADH-dependent reductase, which perfectly fit our expectation——find an efficient, NADH-dependent reductase as our project can only increase the concentration of NADH inside of the cytoplasm. So, to prove our project’s feasibility, this substrate and product will be a perfect model. We introduce this enzyme to our engineered E.coli to catalyze this reaction. We use a promoter pTAC to control the gene CmCR’s expression, which is induced by IPTG.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1353
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 3280
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 3539
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