Composite

Part:BBa_K2242419:Design

Designed by: YongHao Liang   Group: iGEM17_USTC   (2017-10-12)


pTET+ccmA-H


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 534
    Illegal BamHI site found at 3323
    Illegal BamHI site found at 3551
    Illegal BamHI site found at 5895
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 188
    Illegal AgeI site found at 1015
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 4602


Design Notes

No.


Source

The plasmid backbone and the promoter pTET is from the kit plate. The ccm A-H gene is amplified from the genome of a E.coli strain BL21(DE3). The recombinant plasmid is constructed by ourselves.

References