Coding

Part:BBa_K2239012:Design

Designed by: Ruohong Wang   Group: iGEM17_SDSZ-China   (2017-09-29)


7beta-HSDH


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 448
  • 1000
    COMPATIBLE WITH RFC[1000]


Source

The 7beta-HSDH DNA is originally from the genome of R. torques ATCC 35915, and is artificially synthesized based on the sequence retrieved from the GenBank.


References

[1] Ming-Min Zheng, Ru-Feng Wang, Chun-Xiu Li, Jian-He Xu: Two-step enzymatic synthesis of ursodeoxycholic acid with a new 7β-hydroxysteroid dehydrogenase from Ruminococcus torques. Process Biochemistry, Elsevier, 2015.