Part:BBa_K2239008:Design
CBD-LDH
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1266
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 862
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 1122
Design Notes
The CBD tag is added to purify the effective enzymes. When connected with the enzyme DNA and expressed, CBD is able to bind to the cellulose on gauze. After washing off other chemicals in the solution, the enzymes are left on the gauze. As a result, they are successfully immobilized.
Source
The LDH DNA is originally from the genome of Lactobacillus delbrueckii subsp. Bulgaricus, and is artificially synthesized based on the sequence retrieved from the GenBank.
The CBD sequence is retrieved from the GenBank and artificially synthesized.
References
[1] Ming-Min Zheng, Ru-Feng Wang, Chun-Xiu Li, Jian-He Xu: Two-step enzymatic synthesis of ursodeoxycholic acid with a new 7β-hydroxysteroid dehydrogenase from Ruminococcus torques. Process Biochemistry, Elsevier, 2015.
[2] Etai Shpigel, Arie Goldlust, Gilat Efroni, Amos Avraham, Adi Eshel, Mara Dekel, Oded Shoseyov: Immobilization of Recombinant Heparinase I Fused to Cellulose-Binding Domain, 1999.