Coding
Sialidase

Part:BBa_K2235005:Design

Designed by: Shanlin Tong, Shivashree Dhanaraj, Sina Amoor Pour and Gilai Nachmann   Group: iGEM17_Stockholm   (2017-10-11)


Sialidase enzyme


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 55
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 502
    Illegal NgoMIV site found at 577
    Illegal NgoMIV site found at 667
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1047


Design Notes

6xHis-tag is N terminally attached to the enzyme sequence.


Source

References

Egebjerg, J. and Christensen, S. (2005). Cloning, expression and characterization of a sialidase gene from Arthrobacter ureafaciens. Biotechnology and Applied Biochemistry, 41(3), p.225.