Device

Part:BBa_K2233000:Design

Designed by: Kyosuke Kita   Group: iGEM17_Kobe   (2017-10-20)


lacZ for monitoring nasA gene expression in B.subtilis chromosome


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 5376
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The sequence was screened for illegal restriction sites. No illegal restriction site was found.

Source

lacZ and cat (with promoter+RBS) genes were amplified by PCR from commercialized plasmid,pMutin2 and pSweet respectively. Recombination sites sequences (Homologous sequences) were amplified by PCR from B.subtilis strain 168 chromosome. These DNA fragments were assembled together by Gibson assembly.

References