Composite

Part:BBa_K2232022:Design

Designed by: Wenkai Hu   Group: iGEM17_SZU-China   (2017-10-18)


RBS-csoS3-T1


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 74
    Illegal NgoMIV site found at 276
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We add the RBS of B.subtilis and a segment of signal peptide at the upstream of cds of Carboxysomal Carbonic Anhydrase(CsoSCA) from Halothiobacillus neapolitanus，in order to achieve the expression and secretion of this part in the B.subtilis.

Source

This part comes from complete genome of Halothiobacillus neapolitanus c2. GenBank: CP001801.1

References

  • This part was created by 2017 SZU-iGEM team basing on the original part BBa_K1465205.
  • Heinhorst S, Williams E B, Cai F, et al. Characterization of the Carboxysomal Carbonic Anhydrase CsoSCA from Halothiobacillus neapolitanus[J]. Journal of Bacteriology, 2006, 188(23):8087.