Composite

Part:BBa_K2232011:Design

Designed by: Wenkai Hu   Group: iGEM17_SZU-China   (2017-10-18)

RBS_C125-TupA_rrnBT1


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 995
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 815
    Illegal BsaI.rc site found at 1207


Design Notes

We design this part with the rbs of B.subtilis to express in this chassis.

Source

Bacillus halodurans C-125 DNA, complete genome. GenBank: BA000004.3

References

Aono R, Ito M, Machida T. Contribution of the Cell Wall Component Teichuronopeptide, to pH Homeostasis and Alkaliphily in the Alkaliphile, Bacillus lentus C-125[J]. Journal of Bacteriology, 1999, 181(21):6600-6.