Coding

Part:BBa_K2232007:Design

Designed by: Wenkai Hu   Group: iGEM17_SZU-China   (2017-10-18)


BS168-NhaC antiporter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 168


Design Notes

A PstI and a SpeI restriction sites have mutated.

Source

NCBI Reference Sequence: NC_000964.3

References

Prágai Z, Eschevins C, Bron S, et al. Bacillus subtilis NhaC, an Na+/H+ antiporter, influences expression of the phoPR operon and production of alkaline phosphatases.[J]. Journal of Bacteriology, 2001, 183(8):2505.