Coding

Part:BBa_K2232005:Design

Designed by: Wenkai Hu   Group: iGEM17_SZU-China   (2017-10-17)


OF4-CA


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 568


Design Notes

We add a length of sequence encoding signal peptide from Bacillus subtilis to the upstream of the OF4-CA cds,in order to make the protein be secreted out of bacillus subtilis.

Source

GenBank: CP001878.2

References

【1】Tjalsma H, Bolhuis A, Jongbloed J D, et al. Signal peptide-dependent protein transport in Bacillus subtilis: a genome-based survey of the secretome[J]. Microbiology & Molecular Biology Reviews Mmbr, 2000, 64(3):515.

【2】González J M, Fisher S Z. Carbonic anhydrases in industrial applications.[J]. Sub-cellular biochemistry, 2014, 75:405.