Coding

Part:BBa_K2232001:Design

Designed by: Wenkai Hu   Group: iGEM17_SZU-China   (2017-10-17)


OF4-nhaC


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 216
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We express this gene in Bacillus subtilis strain WB800.

Source

GenBank: CP001878.2

References

Ito M, Guffanti A A, Zemsky J, et al. Role of the nhaC-encoded Na+/H+ antiporter of alkaliphilic Bacillus firmus OF4[J]. Journal of Bacteriology, 1997, 179(12):3851-3857.