Generator

Part:BBa_K223052:Design

Designed by: Anusuya Ramasubramanian   Group: iGEM09_Stanford   (2009-10-18)


5MT Mutant TrpR gene


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 1
    Illegal suffix found in sequence at 348
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1
    Illegal SpeI site found at 349
    Illegal PstI site found at 363
    Illegal NotI site found at 7
    Illegal NotI site found at 356
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 1
    Illegal suffix found in sequence at 349
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 1
    Illegal XbaI site found at 16
    Illegal SpeI site found at 349
    Illegal PstI site found at 363
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 195


Design Notes

n/a


Source

Original part taken from GenBank Escherichia coli IAI1: http://www.ncbi.nlm.nih.gov/nuccore/218552585?from=4690442&to=4690768& report=gbwithparts

Two point mutation modifications made to change protein residue at 58 and 79 to Leu and Lys, respectively.

References