Regulatory
Part:BBa_K223044:Design
Designed by: Suzanne Bartram Group: iGEM09_Stanford (2009-10-17)
SoxR - SoxS Promoter System
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal prefix found in sequence at 672
Illegal suffix found in sequence at 506
Illegal SpeI site found at 757
Illegal PstI site found at 771 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 21
Illegal EcoRI site found at 672
Illegal SpeI site found at 507
Illegal SpeI site found at 757
Illegal PstI site found at 521
Illegal PstI site found at 771
Illegal NotI site found at 27
Illegal NotI site found at 514
Illegal NotI site found at 678
Illegal NotI site found at 764 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 21
Illegal EcoRI site found at 672 - 23INCOMPATIBLE WITH RFC[23]Illegal prefix found in sequence at 21
Illegal suffix found in sequence at 507
Illegal EcoRI site found at 672
Illegal XbaI site found at 687
Illegal SpeI site found at 757
Illegal PstI site found at 771 - 25INCOMPATIBLE WITH RFC[25]Illegal prefix found in sequence at 21
Illegal EcoRI site found at 672
Illegal XbaI site found at 36
Illegal XbaI site found at 687
Illegal SpeI site found at 507
Illegal SpeI site found at 757
Illegal PstI site found at 521
Illegal PstI site found at 771 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
Works both on a high and low copy plasmid.
Source
Stanford iGEM team 2009.