Part:BBa_K223035:Design

BLH gene- cleaves beta-carotene into retinoic acid

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal PstI site found at 724
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal PstI site found at 724
  
• 21
  COMPATIBLE WITH RFC[21]
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal PstI site found at 724
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal PstI site found at 724
  
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

BLH gene sequence taken from paper, but altered to get rid of restriction enzyme sites.

Source

Sequence taken from paper

In vitro characterization of a recombinant Blh protein from an uncultured marine bacterium as a β-carotene 15,15′-dioxygenase -Kim et al.

http://www.jbc.org/cgi/reprint/M109.002618v1