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Part:BBa_K223033:Design

Designed by: Suzanne Bartram   Group: iGEM09_Stanford   (2009-07-06)


trp-operon + RBS + IL-6 + HlyA + TT Trp-sensitive IL-6 synthesizer


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 785
    Illegal SpeI site found at 99
    Illegal SpeI site found at 107
    Illegal PstI site found at 572
    Illegal PstI site found at 647
    Illegal PstI site found at 722
    Illegal PstI site found at 743
    Illegal PstI site found at 791
    Illegal PstI site found at 894
    Illegal PstI site found at 927
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 785
    Illegal NheI site found at 704
    Illegal SpeI site found at 99
    Illegal SpeI site found at 107
    Illegal PstI site found at 572
    Illegal PstI site found at 647
    Illegal PstI site found at 722
    Illegal PstI site found at 743
    Illegal PstI site found at 791
    Illegal PstI site found at 894
    Illegal PstI site found at 927
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 785
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 785
    Illegal SpeI site found at 99
    Illegal SpeI site found at 107
    Illegal PstI site found at 572
    Illegal PstI site found at 647
    Illegal PstI site found at 722
    Illegal PstI site found at 743
    Illegal PstI site found at 791
    Illegal PstI site found at 894
    Illegal PstI site found at 927
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 785
    Illegal SpeI site found at 99
    Illegal SpeI site found at 107
    Illegal PstI site found at 572
    Illegal PstI site found at 647
    Illegal PstI site found at 722
    Illegal PstI site found at 743
    Illegal PstI site found at 791
    Illegal PstI site found at 894
    Illegal PstI site found at 927
    Illegal AgeI site found at 271
    Illegal AgeI site found at 536
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 395
    Illegal SapI site found at 809


Design Notes

trp-operon + RBS + IL-6 + HlyA + TT Trp-sensitive IL-6 synthesizer


Source

trp-operon + RBS + IL-6 + HlyA + TT Trp-sensitive IL-6 synthesizer

References