Translational_Unit

Part:BBa_K2230021:Design

Designed by: Yi-Lun Huang   Group: iGEM17_Mingdao   (2017-10-20)


RBS-ptsG/pSB1C3


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 801
    Illegal NgoMIV site found at 1224
    Illegal AgeI site found at 508
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

No


Source

Cloning: The ptsG gene was amplified from gDNA of Salmonella typhimurium using a primer with RBS sequence (B0034), and then cloned onto pSB1C3

References