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Part:BBa_K2228010:Design

Designed by: Caleigh Roleck   Group: iGEM17_Purdue   (2017-10-27)


Benzene Degradation Upper Operon


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The goal of our project was to degrade inhaled carcinogens, so the initial substrate (benzene) must have strong evidence for being a carcinogen and must be commonly inhaled. Further, the final end products of the whole pathway must be incorporated into metabolism. While 2-hydroxymuconate-6-semialdehyde does not incorporate into metabolism, a second pathway can then convert 2-hydroxymuconate-6-semialdehyde to pyruvate and acetyl-CoA


Source

The TetR protein comes from the registry (BBa_C0040). The 4 genes for the benzene-1,2 dioxygenase BnzA, BnzB, BnzC, and BnzD) and the cis-1,2-dihydrobenzene-1,2-diol dehydrogenase gene (BnzE) come from Pseudomonas putida. The catechol-2,3-dioxygenase gene (CatE) comes from Bacillus subtilis.

References