Part:BBa_K2224009
Promoter+RBS+SacB+RBS+GlgC+Terminator （Improved）
This part is the improved version of part BBa_K1600002[1]. We, iGEM2017_SMS_Shenzhen team conducted codon mutation to make sure that this part can meet the standard of RFC[12] and RFC[21].
This composite part was designed to be a sugar polymerization circuit in order to polymerize free simple sugars into their heavy-weight polymers intracellularly. The GlgC gene codes for a protein to polymerize glucose into glycogen. On the other hand, the SacB gene codes for a protein to polymerize fructose into levan and also confers a fructose sensitivity to the chassis. Both components of this part, GlgC and SacB, were studied separately in order to better characterize them. With GlgC alone in a BioBrick device, we found by spectrophotometric analysis that in 10% glucose solution, the E. coli exhibited a 1.5-fold increase in the glycogen production whereas the same BioBrick did not polymerize fructose, as expected in a control. In addition to the glucose polymerization, this bioBrick device demonstrated an ability to inhibit E. coli growth by up to 20% in 10% fructose solution, thereby showing at some evidence of conferred sensitivity due to the SacB gene.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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