Composite

Part:BBa_K2224005

Designed by: Yunchang Zhang   Group: iGEM17_SMS_Shenzhen   (2017-10-24)


SacB promoter RPUs Measurement

Bacillus subtilis subsp. subtilis str. 168 SacB Promotor+RBS+GFP+Terminater This is the standard measurement part of promoter of SacB gene. In our experiment, SacB promoter shows a strong ability in E.coli expression system, contrary to the former belief that this promoter is weak in E.coli.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1205


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