Composite

Part:BBa_K2224001:Design

Designed by: Yunchang Zhang   Group: iGEM17_SMS_Shenzhen   (2017-10-24)


Promoter+RBS+Lecanicillium lecanii strain 432 endochitinase (chit1)+Terminator


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 218
    Illegal NgoMIV site found at 677
    Illegal NgoMIV site found at 884
    Illegal AgeI site found at 786
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

1. Since Lecanicillium lecanii is fungus, the possibility of this chitinase to be expressed and modified successfully in E.coli was unknown. Then, we looked for past scientific papers and found out that there were examples of E.coli expressing this enzyme.*1 2. Since this enzyme is from the Lecanicillium lecanii, a organism that feeds on scale insect, we supposed that this chitinase might shows a strong ability in degradation. We then designed enzyme activity test to get a quantitative analysis of it.


Source

Lecanicillium lecanii strain 432

References