Composite

Part:BBa_K2223004:Design

Designed by: Thomas Sousa   Group: iGEM17_UCSC   (2017-11-01)


B_12 Riboswitch


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 392
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The sequence had to be codon optimized for use in Synechococcus elongatus PCC 7942. Homologous arms were added later for recombination into the genome of S. elongatus PCC 7942.


A light-induced psbAI (Part:BBa_K1913011) promoter, documented to be constitutively expressed in cyanobacteria such as S. elongatus PCC 7942, was modified at the -10 element for expression in E. coli. The promoter was placed upstream of the biobrick parts, producing two separate mRNA strands. The reported paired with this is documented in https://parts.igem.org/Part:BBa_K2223005

Source

Synechococcus Elongatus (Part: BBa_K754000), Escherichia coli(Part:BBa_K1913011),

References