Coding

Part:BBa_K2220019:Design

Designed by: Nan Rong   Group: iGEM17_BNU-China   (2017-10-22)


Display alpha-tubulin protein


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 325
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 388
    Illegal XhoI site found at 1747
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1816
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 784
    Illegal BsaI site found at 1798
    Illegal BsaI.rc site found at 1490
    Illegal SapI site found at 648


Design Notes

We have done the codon optimization.


Source

Aga2 comes from pYD1 plasmid. Alpha-tubuin: http://www.ncbi.nlm.nih.gov/nuccore/393715088

References