Coding

Part:BBa_K2220013:Design

Designed by: Nan Rong   Group: iGEM17_BNU-China   (2017-10-22)


Secrete FliC(EG) fusion protein


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We have done the codon optimization.


Source

Alpha-factor comes from pyes2/ct/alpha-factor plasmid. EG: https://www.ncbi.nlm.nih.gov/protein/1134776103?report=genbank&log$=protalign&blast_rank=1&RID=PV4FEB9F015 FliC comes from E.coli K12.


References