Coding

Part:BBa_K2220010:Experience

Designed by: Nan Rong   Group: iGEM17_BNU-China   (2017-10-21)


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Applications of BBa_K2220010

We ligated this part to shuttle plasmid pYES2/CT/α-factor(pYCα) and did the colony PCR to verify the efficiency(Fig.1). Meanwhile, the sequencing results further confirmed that we successfully cloned the expression vectors.

Fig.1 Electrophoresis result of BBa_K2220010 expression vector.

We successfully transfected the correct carrier into S.cerevisiae INVSc1 and induced expression.

Fig.2 Fluorescence image of recombinant FliC(eGFP) expressed by the engineered yeast cell.

Here are the protocols we used.

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