Coding

Part:BBa_K2220005:Design

Designed by: Nan Rong   Group: iGEM17_BNU-China   (2017-10-18)


Display PETase protein


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 325
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 472
    Illegal BamHI site found at 388
    Illegal XhoI site found at 1186
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1255
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1237


Design Notes

we have done the codon optimization.


Source

https://www.ncbi.nlm.nih.gov/protein/928998053?report=genbank&log$=protalign&blast_rank=1&RID=X3B87DWV014

References