Coding

Part:BBa_K2215001

Designed by: Megan Sare   Group: iGEM17_ULaVerne_Collab   (2017-08-21)


FeSOD Clade A

Superoxide dismutases (SOD) are the first line of defense against reactive oxygen species when cells become stressed. This makes them important antioxidants in the cell that is responsible for converting superoxide anion radicals into hydrogen peroxide, which can be further converted into water. (Alscher et al., 2002) There are different types SODs, but this gene is iron superoxide dismutase (FeSOD), which resides in the chloroplast. The sequence for this SOD came from a Clade A Symbiodinium, which is one of the least heat resistant clades. This FeSOD is characterized in Chlamydomonas and should therefore be used in Chlamydomonas or can be codon optimized for other species.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 145
    Illegal NgoMIV site found at 524
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 64


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Categories
Parameters
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