Translational_Unit

Part:BBa_K2213013:Design

Designed by: Adam Hannaford   Group: iGEM17_Manchester   (2017-10-10)


araB_eutLK_LowPromoter_PduD(1-20)_mCherry_cgPPK2


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1300
    Illegal NheI site found at 2701
    Illegal NheI site found at 2724
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1239
    Illegal BamHI site found at 2164
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1074
    Illegal AgeI site found at 1775
    Illegal AgeI site found at 1952
    Illegal AgeI site found at 2252
    Illegal AgeI site found at 2315
    Illegal AgeI site found at 2362
    Illegal AgeI site found at 3601
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 2839
    Illegal SapI site found at 1056
    Illegal SapI.rc site found at 4280


Design Notes

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Source

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References